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Exon 1 (transcript variant 3) and exon 10 were amplified from selected samples with PCR parameters as described above. After a control electrophoresis on 2% agarose gels the PCR products were directly subjected to processing with Surveyor nuclease [13] (Transgenomic, Omaha, USA) according to the protocol supplied by the manufacturer: PCR products from a reference sequence were mixed at equimolar amounts with wild type samples, denatured at 95°C for 2 minutes and subsequently re-hybridized. In case of sequence deviations in the wildtype samples from the reference sequence this resulted in the formation of heteroduplexes containing mismatches. In the following incubation with Surveyor nuclease for 20 min. at 42°C heteroduplexes were cleaved at mismatch sites by the enzyme leading to DNA fragments with reduced length. These digestion products were subjected to electrophoresis on 2% agarose gels..

The ethics committee of the NTUH approved the current study (NO. 201212165RINC). The local institutional review board did not mandate patient consent, since no interventions were made and patient privacy was not breached.. In the treatment of HCV, the benefits of peg interferon alpha and ribavirin have become clear over the past decade. Where on one hand, this treatment has proven its effectiveness in ideal population; it has also identified some special populations by way of poor response or difficulties faced due to co morbid conditions. These special populations include patients with cirrhosis, non responders to prior treatment, HIV positive patients, patients with African American ethnicity, steatosis, and post liver transplants.

In the treatment of HCV, the benefits of peg interferon alpha and ribavirin have become clear over the past decade. Where on one hand, this treatment has proven its effectiveness in ideal population; it has also identified some special populations by way of poor response or difficulties faced due to co morbid conditions. These special populations include patients with cirrhosis, non responders to prior treatment, HIV positive patients, patients with African American ethnicity, steatosis, and post liver transplants..

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